Background: To discuss the reliability and clinical value of Icon port, a Self-made single port device, which was applied for transumbilical single port laparoscopic appendectomy. Methods: The incision was around 2.5 cm long with curved form around umbilicus. And the skin and each layer of the abdominal wall were cut in sequence. Then the silica gel sleeve of the self-made single-incision sealing device was folded, clamped by the forceps, and carefully inserted into the incision for sealing. The operation of laparoscopic appendectomy was accomplished by-use routine line type laparoscopic instruments via Icon port. The specimen was discharged from peritoneal cavity through the single port device. Two layers of incision, linea alba abdominus and skin, were separately sutured by absorbable thread and each layer was sutured successively. Results: Among the 272 patients, 264 were successfully performed single-port laparoscopic appendectomy, with the operation time ranging from 30 to 110 min (69.34±21.4 min on average). In 8 cases, auxiliary operating hole was added or laparotomy was performed. Total hospital stay was 4.8±1.8 (2-14) days. 6 cases of incision infection. All patients were followed up for 1 to 6 months after surgery, with an average of 3 months. There were no complications such as bleeding, intestinal leakage, intestinal obstruction, and residual peritoneal effusion. Conclusion: Icon port single-hole device, which has potential promotion value, can be used in relatively simple laparoscopic surgery alone or combined with a single puncture device for relatively complex laparoscopic surgery. Trial registration: The study was registered with the Chinese Clinical Trial Registry (ChiCTR)(www.chictr.org.cn). Registration number: ChiCTR1900023056. Registration date: May 9, 2019.Retrospectively registered.