To explore the predictive value and correlation analysis of red blood cell volume distribution width  (RDW) combined with plasma albumin and amylase (AMY) indexes in patients with acute pancreatitis (AP) in emergency department.Methods:A total of 158 patients with acute pancreatitis admitted to our hospital from June 2020 to June 2021 were selected as the case group, and 140 healthy volunteers who underwent physical examination in our hospital were randomly selected as the control group. RDW, plasma albumin and AMY were detected by real-time PCR. The expression and significance of RDW, plasma albumin and AMY in acute pancreatitis were analyzed.Results:RDW and AMY expressions in case group were significantly higher than those in control group, and plasma albumin expression was significantly lower than that in control group, with statistical difference (P < 0.05). Compared with mildly infected patients, RDW and AMY expressions were higher and plasma albumin expression was lower in severely infected patients, with statistical difference (P < 0.05). With the aggravation of acute pancreatitis, the expression of RDW and AMY increased, while the expression of plasma albumin decreased, with statistical difference (P < 0.05). The diagnostic value of RDW, plasma albumin and AMY was higher than that of RDW, plasma albumin and Amy, with statistical difference (P < 0.05). Plasma albumin and AMY showed a negative correlation (r=-5.978, p=0.015). Plasma albumin and RDW showed a positive correlation (r=4.054, p=0.045). AMY and RDW showed a negative correlation (r=-4.402, p=0.037).Conclusion:The detection of RDW, plasma albumin and AMY showed that RDW and AMY showed high expression in acute pancreatitis, while plasma albumin showed low expression, and the changes of the above indicators can be used in clinical early assessment of acute pancreatitis.