Objective To investigate how safety nursing in the operating room affects patients' post-gynecological surgery anxiety and sadness. Methods Retrospective analysis was done on the clinical information of 102 patients who underwent laparoscopic surgery at our hospital's gynecological department between July 2020 and May 2022. Using a random number table, they were separated into two groups with 51 instances each: a controlling group and an observational group. The observational team received safety nurse intervention in the operating room, whereas the controlling team received normal nursing assistance. The duration of the two groups' operations, intraoperative blood loss, hospital stays, and times required for ambulation and bowel sound recovery were tracked. The levels of serum cortisol (Cor) and high-sensitivity C-reactive protein (hs-CRP), the ratings of life quality before and after intervention, and the visual analogue scale (VAS) at 1h, 2h, 3h, and 4h after surgery were tested in two teams. Additionally, the two groups' nurse satisfaction levels were contrasted. Results When compared to the controlling group, the observation team's operation time, ambulation time, recovery time, and intraoperative blood loss for bowel sounds, and hospital stay were all considerably shorter (P< 0.05). The SAS and SDS grades in the two teams were considerably lower than before the interfere (P<  0.05); and following the intervention, the observational team's SAS and SDS grades were substantially lower than the controlling team's (P< 0.05). At 1h, 2h, 3h, and 4h after the surgery, the VAS grade in the observational team was substantially lower than that in the controlling team following the intervention (P< 0.05). The levels of serum Cor and hs-CRP in the two teams were significantly lower after the treatment when compared to before it, and in the observational team, they were meaningfully lower when confronted with those in the controlling team (P<0.05). The grades for vigor, sentimental function, social function, somatic pain, and physical function, in the two categories were considerably higher after the treatment compared to before it (P< 0.05), and the grades for sentimental function, physical function, vigor, somatic pain, and social function in the observational team were considerably higher after the intervention compared to those in the controlling team. 30 instances in the observational group were extremely happy, 19 instances were usually satisfied, and 96.07% of the instances were completely satisfied. In the controlling group, there were 25 instances that were usually satisfied and 15 instances that were extremely satisfied, for a satisfaction rate of 78.43%. Additionally, there was a considerable distinction in the two teams' overall satisfaction (P<0.05). Conclusion It is important to spread the word about safety nursing in operating rooms because it can considerably reduce the amount of time patients with gynecological surgery spend in surgical procedure, ease their depression symptoms, lessen their body's stress response, strengthen their level of pain, and improve their quality of life. These benefits also contribute to the development of a strong nurse-patient connection and help patients recover faster.