The Study of Phenolics from Telosma cordata (Burm. f.) Merr. flowers as α-Glucosidase Inhibitors: In-Vitro Assessment and Molecular Docking

Authors

  • Shuai Wang School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Chen Tang School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Fangfang Luo School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Yingying Shao School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Jinmei Lei School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Chen Lu School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Li Chen School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Tingting Li School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China
  • Xudong Jiang School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

Keywords:

Telosma cordata (Burm. f.) Merr. flowers; UPLC-MS/MS; antioxidant; a-glucosidase inhibition; molecular docking

Abstract

Telosma cordata (Burm. f.) Merr. flowers (TBMF) serve as food and has several medicinal uses in southern China. The current study investigated the phenolic ingredients, antioxidant ac-tivity, α-glucosidase and cell growth inhibitory activity of ethanol extract of TBMF in vitro. The in silico binding mechanisms of these phenolic compounds with α-glucosidase were elucidated via autodock vina software. The results showed that TBMF contained a content of total phenolic with the value of 40.51 ± 0.72 mg GAE/g. The TBMF extract exhibited significant antioxidant ac-tivities (ABTS•+ and DPPH• scavenging capacities) with IC50 values of 82.51± 0.98 and 15.21 ± 0.31 μg/mL, respectively. It also displayed excellent α-glucosidase inhibitory activity with the IC50 value of 71.05 ± 0.65 μg/mL, the kinetic experiments showed that the inhibition type on α-glucosidase belonged to the mixed type. In addition, twenty-six phenolic compounds were identified and quantified from TBMF extract by UPLC-MS/MS including phenolic acids, flavo-noids, polyphenols and benzoic acid derivatives. Furthermore, molecular docking has revealed that most identified phenolic compounds have low binding energy at their characteristic bind-ing activity sites in α-glucosidase, which indicated the possible roles of these phytochemicals in enzyme inhibitory activities. These results indicated that TBMF had significant antioxidant and excellent α-glucosidase inhibitory activities, which could provide theoretical basis for the re-search and development of it as antioxidant and hypoglycemic food.

 

Author Biographies

Shuai Wang, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

Chen Tang, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

Fangfang Luo, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

 

 

 

 

Yingying Shao, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

Jinmei Lei, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

Chen Lu, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

Tingting Li, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

 

Xudong Jiang, School of Medicine, Guangxi University of Science and Technology, 545005 Liuzhou, China

 

 

 

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Published

2023-04-04