Güliz Şenormancı, Çetin Turan
Department of Psychiatry, University of Health Sciences Bursa Yuksek Ihtisas Training and Research Hospital, Bursa, Turkey
Sevim Karakaş Çelik
Department of Medical Genetics, School of Medicine, Zonguldak Bulent Ecevit University, Zonguldak, Turkey
Aycan Çelik
Department of Molecular Biology and Genetics, Faculty of Sciences and Arts, Zonguldak Bülent Ecevit University, Zonguldak, Turkey
Tuba Gökdoğan Edgünlü
Department of Medical Biology, School of Medicine, Muğla Sıtkı Koçman University, Muğla, Turkey
Ceren Bilgi
Department of Molecular Biology and Genetics, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, Turkey
Ayşe Semra Demir Akca
Department of Family Medicine, School of Medicine, Zonguldak Bülent Ecevit University, Zonguldak, Turkey
Ömer Şenormancı
Department of Psychiatry, University of Health Sciences Bursa Yuksek Ihtisas Training and Research Hospital, Bursa, Turkey
Abstract:
Background: Exocytosis-related gene variants have been suggested to be associated with externalizing behaviors. Objective: This study aimed to examine VAMP2 26 bp Ins\Del, synaptotagmin XI (Syt11) rs3820594 and 33-bp promoter, Syntaxin 1A (Syn-1A) rs1569061 and SNAP-25 rs1051312 and rs3746544 polymorphisms, their serum levels and their relationship with impulsivity, temperament in individuals with alcohol dependence (AD) and healthy controls (HC). Methods: The study included 107 individuals with AD and 104 HCs. Single-nucleotide polymorphisms (SNPs) were studied with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method and serum levels with ELISA. Michigan Alcohol Screening Test (MAST), Barratt Impulsiveness Scale-11 (BIS-11) and Temperament Evaluation of Memphis, Pisa, Paris and San Diego Autoquestionnaire (TEMPS-A) were applied. Results: Syn-1A rs1569061 C allele polymorphism was significantly higher in AD group. Syn-1A rs1569061 C allele was associated with 1.5 times increased risk of AD. All serum levels were significantly higher in the HC group. There was a relationship between Syn-1A rs1569061 polymorphism and BIS-11 motor impulsiveness in the AD group; Syt11 rs3820594 polymorphism and BIS-11 total, TEMPS-A depressive, hyperthymia in the HC group. Discussion: In our study, gene variants and serum levels of synaptic vesicle and presynaptic plasma membrane proteins were related to AD, impulsivity and temperament.
Keywords:Alcohol dependence, VAMP2, Synaptotagmin XI, Syntaxin 1A, SNAP-25.