Background: Contextual factors involving the physical and social environment, as well as personal factors, are closely related to functional status, and they can have positive or negative influences on the health conditions or status of an individual in society. Objectives: The objective is to evaluate the effect of a mindfulness-based intervention program on functional status and mindfulness levels in primary health care (PHC) professionals in Ribeirão Preto, Brazil. Methods: This is a quasi-experimental study, with 26 PHC professionals, using quantitative methods and an analytical before and after approach of an 8-week mindfulness program. Results: There were significant differences in mindfulness facets after the intervention: Observe (p = 0.002); Describe – positive formulation (p = 0.01); Acting with awareness – automatic pilot (p = 0.01) and distraction (p = 0.05); Nonreactivity (p = 0.0005); Nonjudgement (p = 0.01); and in total mindfulness scores (p = 0.0000018). Regarding functional status, significant differences were found: change in health (p = 0.01), overall health (p = 0.007), quality of life (p = 0.04) and feelings (p = 0.01). Discussion: The results in improving the functional status and mindfulness of PHC professionals show that mindfulness practices can improve the worker’s quality of life and health.